TB-500 Dosaging Guide
Research Disclaimer Content on this page is for research and educational purposes only. BioLabDirect products are sold as research chemicals and are not for human or veterinary use, diagnosis, treatment, or disease prevention. Nothing here is medical advice, a dosing protocol, or a clinical claim. Always follow institutional lab policies and applicable regulations.
For Research Purposes Only.
This TB-500 dosaging guide gives laboratory researchers the practical pieces they need before a bench experiment: what TB-500 is and is not, the reconstitution and concentration arithmetic with a worked table, storage notes, and a checklist for reading the Certificate of Analysis. It does not provide instructions for use in people or animals and makes no medical claims. Browse the full research peptide catalog for other materials.
Internal resources:
- TB-500 research peptide (10mg vial)
- Peptide reconstitution calculator
- COA library (third-party lab reports)
- BPC-157 Dosaging Guide · GHK-Cu Dosaging Guide
- Related research peptides: BPC-157 · GHK-Cu
TB-500 and Thymosin β4: What the Sources Say
TB-500 is a distinct synthetic peptide. Suppliers describe it as a synthetic fragment related to thymosin β4 (Tβ4); it is not the full-length Tβ4 protein. This matters for reading the literature: the studies cited on this page concern thymosin β4, not TB-500 itself, and none of them tested TB-500. They are listed only as background on the parent molecule.
Goldstein et al. (2005) review Tβ4 and describe it as an actin-sequestering protein. Philp et al. (2004) is a short paper reporting that Tβ4 altered angiogenesis and cell-migration readouts in rodent experimental models; it is cited here for background on the parent molecule only.
| Item | Detail for lab planning |
|---|---|
| Name | TB-500 (supplier name; synthetic peptide related to thymosin β4) |
| Form supplied | Lyophilized powder, 10 mg vial |
| Sequence and mass | Take from the lot CoA; do not assume the full-length Tβ4 values |
Thymosin β4 Themes in the Published Literature
- Protein biochemistry: Goldstein et al. (2005) describe Tβ4 as an actin-sequestering protein.
- Experimental models: Goldstein et al. (2005) summarize preclinical work on Tβ4 across several experimental model systems; see the review for the specific models and endpoints.
- Rodent readouts: Philp et al. (2004) report angiogenesis and cell-migration readouts in rodent models.
These are descriptions of study findings on the parent molecule. They are not statements about TB-500 or any BioLabDirect product.
Reconstitution and Concentration Math for Laboratory Research
For lyophilized TB-500, concentration is the labelled mass divided by the diluent volume, and the volume you draw for an assay is the target mass divided by that concentration. Convert units first (1 mg = 1,000 µg) and use the net peptide content from the lot CoA. The peptide reconstitution calculator automates the same steps.
| Step | Calculation | Result |
|---|---|---|
| 1. Vial content | Label amount | 10 mg of TB-500 |
| 2. Diluent added | Chosen by the lab for the assay | 2 mL |
| 3. Stock concentration | 10 mg ÷ 2 mL | 5 mg/mL (5,000 µg/mL) |
| 4. Example working aliquot | 250 µg ÷ 5,000 µg/mL | 0.05 mL (50 µL) |
The 250 µg figure is an arbitrary arithmetic illustration, not an amount drawn from any study and not a recommendation. Doubling the diluent volume halves the concentration, so always record the volume used.
What the Published Literature Does and Doesn’t Specify
Thymosin β4 papers express amounts in model-specific ways (per animal, per body weight, per area, or per milliliter of medium), and because they study the full-length protein, those values cannot be carried over to TB-500. This guide therefore lists no numerical amounts. For experimental design, read the methods sections of the primary papers listed below, apply your institution’s approvals, and use the calculator to turn your chosen target into volumes.
Storage and Handling for a 10 mg TB-500 Vial
- Keep unopened vials sealed, cold, dry and light-protected as the lot CoA directs.
- After reconstitution, split the stock into single-use aliquots. At 5 mg/mL, a 50 µL aliquot holds 250 µg, so one vial yields about 40 such aliquots from 2 mL.
- Label every tube with date, concentration and diluent, and avoid repeated freeze–thaw cycles.
- Peptide stability depends on sequence, pH, temperature and buffer; Manning et al. (2010) review the general principles. Confirm stability under your own assay conditions.
TB-500 vs Thymosin β4: What the CoA Should Show
Because TB-500 and full-length Tβ4 are different molecules, the CoA is where you confirm which one you hold. Check that:
- the lot number on the vial matches the report;
- the observed mass (mass spectrometry) matches the sequence the supplier states for TB-500, not the mass of full-length Tβ4;
- HPLC purity is reported with the chromatogram, along with the test date and testing laboratory.
Lot reports are in the COA library; compare yours with the matching entry. Ready to plan your next run? See the TB-500 research peptide product page.
Frequently Asked Questions
What is TB-500?
TB-500 is the supplier name for a synthetic peptide described as a fragment related to thymosin β4. It is a distinct molecule from full-length thymosin β4, so confirm its sequence and mass on the lot CoA.
Do the cited studies test TB-500?
No. The cited papers study thymosin β4. They are included as background on the parent molecule and do not establish anything about TB-500 itself.
How do I work out the concentration of a reconstituted 10 mg vial?
Divide the mass by the diluent volume: 10 mg in 2 mL is 5 mg/mL (5,000 µg/mL). The calculator handles other volumes. This is laboratory arithmetic only.
Why does this guide list no numerical amounts?
Published thymosin β4 papers use model-specific units and study the full-length protein, so their values do not transfer to TB-500. Nothing here is guidance for use in people or animals.
How can I tell whether my lot is what the label says?
Match the lot number to the report in the COA library and compare the observed mass with the stated TB-500 sequence.
References
Please read each full paper before relying on any detail.
- Goldstein AL, Hannappel E, Kleinman HK. Thymosin β4: actin-sequestering protein moonlights to repair injured tissues. Trends Mol Med. 2005;11(9):421–429. PMID: 16099219. DOI: 10.1016/j.molmed.2005.07.004.
- Philp D, Kleinman HK, et al. Thymosin β4 promotes angiogenesis, wound healing, and hair follicle development. Mech Ageing Dev. 2004;125(2):113–115. PMID: 15037013. DOI: 10.1016/j.mad.2003.11.005.
- Manning MC, Chou DK, Murphy BM, Payne RW, Katayama DS. Stability of protein pharmaceuticals: an update. Pharm Res. 2010;27(4):544–575. PMID: 20143256. DOI: 10.1007/s11095-009-0045-6.
Research Disclaimer This article is educational only. BioLabDirect sells research chemicals not intended for human consumption or clinical use. No statement here establishes safety or effectiveness for any condition, and nothing here is a recommendation for any person or animal. Follow institutional biosafety rules.
For Research Purposes Only.
